梨形四膜虫微管蛋白的合成位点。
Site of tubulin synthesis in Tetrahymena pyriformis.
作者信息
Barahona I, Rodrigues-Pousada C
出版信息
FEBS Lett. 1983 Jul 25;158(2):271-5. doi: 10.1016/0014-5793(83)80593-6.
Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the alpha- and beta-tubulin subunits.
从梨形四膜虫这种原生动物中分离出游离多核糖体和膜结合多核糖体，并在兔网织红细胞裂解物中利用35S翻译产物的免疫沉淀法研究了这两种多核糖体对微管蛋白合成的贡献。一维电泳分析表明，微管蛋白是由从游离多核糖体和膜结合多核糖体中分离出的多聚腺苷酸化RNA合成的。游离多核糖体的非多聚腺苷酸化RNA也能够指导微管蛋白的合成。使用奥法雷尔系统进行的二维电泳分析证实了这些结果，并且还揭示了α-和β-微管蛋白亚基的存在。
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