Fliss E R, Suyama Y
J Protozool. 1979 Aug;26(3):505-9. doi: 10.1111/j.1550-7408.1979.tb04661.x.
Tetrahymena outer doublet tubulin was compared with neurotubulin and Chlamydomonas flagellar tubulin on SDS-polyacrylamide gels. Tetrahymena alpha tubulin did not comigrate with either brain or flagellar alpha tubulins, although brain, flagellar, and ciliary beta tubulins all comigrated. Axonemal tubulin from Tetrahymena strain ST was compared with this tubulin from strains W, S, HSM, and E, and all were found to have the same mobilities. Poly-A containing RNA was separated from whole cell Tetrahymena RNA by oligo-dT cellulose chromatography. Poly-A+ RNA from 24-h cultures (early exponential growth) stimulated greater incorporation of amino acids into polypeptides in the wheat germ cell-free translation system than did poly-A+ RNA from 36-h and 49-h cultures. When separated on SDS-polyacrylamide gels, the translation products of the 24-h poly-A+ RNA had 2 prominent protein bands which comigrated with alpha and beta tubulin isolated from Tetrahymena cilia. These bands were not found in the translation products of poly-A+ RNA isolated from 49-h cultures or in the translation products of poly-A- RNA.
在十二烷基硫酸钠-聚丙烯酰胺凝胶上，将四膜虫外双联体微管蛋白与神经微管蛋白和衣藻鞭毛微管蛋白进行了比较。四膜虫α微管蛋白与脑或鞭毛α微管蛋白均未共迁移，尽管脑、鞭毛和纤毛β微管蛋白都发生了共迁移。将四膜虫ST株的轴丝微管蛋白与W、S、HSM和E株的这种微管蛋白进行了比较，发现它们的迁移率相同。通过寡聚-dT纤维素层析从四膜虫全细胞RNA中分离出含聚腺苷酸的RNA。在小麦胚无细胞翻译系统中，来自24小时培养物（早期指数生长）的聚腺苷酸+RNA比来自36小时和49小时培养物的聚腺苷酸+RNA刺激氨基酸更多地掺入多肽中。当在十二烷基硫酸钠-聚丙烯酰胺凝胶上分离时，24小时聚腺苷酸+RNA的翻译产物有两条明显的蛋白带，它们与从四膜虫纤毛中分离出的α和β微管蛋白共迁移。在从49小时培养物中分离出的聚腺苷酸+RNA的翻译产物或聚腺苷酸-RNA的翻译产物中未发现这些条带。
