Shousha S, Mitchell T R
Histochem J. 1983 Jun;15(6):563-9. doi: 10.1007/BF01954147.
An Orcein staining method has been developed which stains mature and immature leukocytes in blood films and bone-marrow smears. Two different patterns of staining are obtained depending upon whether staining is or is not preceded by oxidation. In the latter case, all granulocytes and some monocytes show granular reddish-brown cytoplasmic staining. When prior oxidation is used, the staining is in the form of fine grey or black cytoplasmic granules. All lymphocytes, by both techniques, are negative. It is suggested that Orcein stains sulphated mucosubstances, possibly chondroitin sulphate, which in granulocytes is concentrated in their primary granules.
已开发出一种地衣红染色法，可对血涂片和骨髓涂片中的成熟和未成熟白细胞进行染色。根据染色前是否进行氧化，可获得两种不同的染色模式。在后一种情况下，所有粒细胞和一些单核细胞显示出颗粒状红棕色细胞质染色。当使用预先氧化时，染色呈细灰色或黑色细胞质颗粒形式。通过这两种技术，所有淋巴细胞均为阴性。有人认为地衣红可染色硫酸化粘多糖物质，可能是硫酸软骨素，在粒细胞中它集中在其初级颗粒中。
