Gaffney D, Skurray R, Willetts N
J Mol Biol. 1983 Jul 25;168(1):103-22. doi: 10.1016/s0022-2836(83)80325-8.
Hybridization experiments and tra-lacZ fusions were used to obtain further insight into the complex series of control systems that affect F conjugation. We confirmed that the regular IncF FinOP control system represses transcription of traJ, and found that the traJ product is required for transcription of traM as well as of the traY-Z operon. The chromosomal sfrA gene product may be required to prevent premature termination of traJ transcription, while the sfrB gene product prevents premature termination at two sites within the traY-Z operon. The FinQ inhibition system determined by several IncI plasmids caused termination at three different sites in the operon, and that of JR66a at one further site. JR66a and R485 strongly inhibit F transfer, but have weak, or no (respectively) effects on transcription: they may inhibit function of one or more transfer gene products.
杂交实验和tra-lacZ融合技术被用于进一步深入了解影响F接合的复杂控制系统系列。我们证实了常规的IncF FinOP控制系统会抑制traJ的转录，并且发现traJ产物是traM以及traY-Z操纵子转录所必需的。染色体上的sfrA基因产物可能是防止traJ转录过早终止所必需的，而sfrB基因产物则可防止traY-Z操纵子内两个位点的过早终止。由几种IncI质粒决定的FinQ抑制系统会导致操纵子在三个不同位点终止转录，而JR66a的抑制系统会导致在另外一个位点终止转录。JR66a和R485强烈抑制F转移，但对转录的影响较弱或无影响（分别对应）：它们可能抑制一种或多种转移基因产物的功能。
