McKeon F D, Tuffanelli D L, Fukuyama K, Kirschner M W
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4374-8. doi: 10.1073/pnas.80.14.4374.
We have studied the autoantibodies in the serum of a patient with linear scleroderma that specifically recognize the nuclear envelope of cultured cells. These antibodies bind to conserved determinants of nuclear lamins, the predominant mammalian nuclear envelope proteins. Of the three mammalian nuclear lamin proteins (970, P68, and P60), only P70 and P60 bind the autoantibodies. In addition, two proteins of the Drosophila embryonic nuclear matrix, P70 and P68, bind these autoantibodies. We have used nuclear matrices to isolate the autoantibodies from the patient's serum that react to the nuclear lamins. At least three different IgG heavy chains were found to be involved in this autoimmune response to nuclear lamins, indicating that this response is not due to the expansion of a single B-cell clone.
我们研究了一名线性硬皮病患者血清中的自身抗体，这些抗体能特异性识别培养细胞的核膜。这些抗体与核纤层蛋白的保守决定簇结合，核纤层蛋白是哺乳动物核膜的主要蛋白质。在三种哺乳动物核纤层蛋白（970、P68和P60）中，只有P70和P60能结合自身抗体。此外，果蝇胚胎核基质的两种蛋白质P70和P68也能结合这些自身抗体。我们利用核基质从患者血清中分离出与核纤层蛋白发生反应的自身抗体。发现至少三种不同的IgG重链参与了对核纤层蛋白的这种自身免疫反应，这表明这种反应并非由单个B细胞克隆的扩增所致。
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