烟酰胺腺嘌呤二核苷酸糖水解酶，一种小牛脾细胞的胞外酶。
NAD+ glycohydrolase, an ecto-enzyme of calf spleen cells.
作者信息
Muller H M, Muller C D, Schuber F
出版信息
Biochem J. 1983 May 15;212(2):459-64. doi: 10.1042/bj2120459.
Abstract
By using a sensitive fluorimetric assay of NAD+ glycohydrolase (EC 3.2.2.6), we showed that calf spleen cells are able to hydrolyse 1,N6-etheno-NAD+ given in the medium. The observed rates of substrate hydrolysis and product accumulation in the medium are equivalent. Moreover, the splenocytes are able to cleave the nicotinamide-ribose bond of a water-soluble polymer of NAD+, and their NAD+ glycohydrolase activity is fully inhibited by a high-molecular-weight Blue Dextran. Selective permeation of the cellular membrane digitonin revealed an intracellular pool of NAD+ glycohydrolase, which accounts for 25% of the total activity. We conclude that NAD+ glycohydrolase associated with the splenocytes has the characteristics of an ecto-enzyme.
摘要
通过使用灵敏的NAD⁺糖水解酶（EC 3.2.2.6）荧光测定法，我们发现小牛脾细胞能够水解培养基中添加的1,N⁶-乙烯基-NAD⁺。观察到的底物水解速率和培养基中产物积累速率相当。此外，脾细胞能够裂解NAD⁺水溶性聚合物的烟酰胺-核糖键，其NAD⁺糖水解酶活性被高分子量蓝色葡聚糖完全抑制。细胞膜洋地黄皂苷的选择性通透揭示了NAD⁺糖水解酶的细胞内池，其占总活性的25%。我们得出结论，与脾细胞相关的NAD⁺糖水解酶具有胞外酶的特征。
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