人卵黄囊瘤中产生的甲胎蛋白糖链。

Sugar chain of alpha-fetoprotein produced in human yolk sac tumor.

作者信息

Yamashita K, Hitoi A, Tsuchida Y, Nishi S, Kobata A

出版信息

Cancer Res. 1983 Oct;43(10):4691-5.

PMID:6192908

Abstract

The carbohydrate moiety of alpha-fetoprotein purified from human yolk sac tumors grown in nude mice was quantitatively released from the polypeptide chain as oligosaccharides by hydrazinolysis. The oligosaccharides were separated into a neutral and two acidic oligosaccharides by paper electrophoresis. By sequential exoglycosidase digestion in combination with per-O-methylation study and periodate oxidation, their structures were determined to be: Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(GlcNAc beta 1 leads to 4) (Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4(Fuc alpha 1 leads to 6)GlcNAcOT, Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(GlcNAc beta 1 leads to 4) (Sia alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4(Fuc alpha 1 leads to 6)GlcNAcOT; and Sia alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(GlcNAc beta 1 leads to 4) (Sia alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4 GlcNAc beta 1 leads to 4(Fuc alpha 1 leads to 6)GlcNAcOT, in which Gal is galactose, GlcNac is N-acetylglucosamine, Man is mannose, Fuc is fucose, Sia is sialic acid, and Subscript OT is the NaB3H4-reduced oligosaccharide.

摘要

从在裸鼠体内生长的人卵黄囊瘤中纯化得到的甲胎蛋白的碳水化合物部分，通过肼解从多肽链上以寡糖的形式定量释放出来。通过纸电泳将这些寡糖分离为一种中性寡糖和两种酸性寡糖。通过外切糖苷酶顺序消化并结合全O-甲基化研究和高碘酸盐氧化，确定它们的结构为：Galβ1→4GlcNAcβ1→2Manα1→6(GlcNAcβ1→4)(Galβ1→4GlcNAcβ1→2Manα1→3)Manβ1→4GlcNAcβ1→4(Fucα1→6)GlcNAcOT、Galβ1→4GlcNAcβ1→2Manα1→6(GlcNAcβ1→4)(Siaα2→6Galβ1→4GlcNAcβ1→2Manα1→3)Manβ1→4GlcNAcβ1→4(Fucα1→6)GlcNAcOT；以及Siaα2→6Galβ1→4GlcNAcβ1→2Manα1→6(GlcNAcβ1→4)(Siaα2→6Galβ1→4GlcNAcβ1→2Manα1→3)Manβ1→4GlcNAcβ1→4(Fucα1→6)GlcNAcOT，其中Gal是半乳糖，GlcNac是N-乙酰葡糖胺，Man是甘露糖，Fuc是岩藻糖，Sia是唾液酸，下标OT是用NaB3H4还原的寡糖。