Valenzuela J E, Lamers C B, Modlin I M, Walsh J H
Gut. 1983 Sep;24(9):807-11. doi: 10.1136/gut.24.9.807.
To determine the role of cholinergic reflexes on pancreatic secretory response to food, we studied the effect of atropine on amylase secretion in response to the octapeptide of cholecystokinin (CCK8) and to intraintestinal oleate. Four studies were done in six healthy volunteers. The duodenal content was aspirated by a double lumen tube while synthetic secretin (41 pmol/kg/h) was infused as a background in all the studies. Graded doses of CCK8 IV or 0.42 M oleate pH 9.4 at 25 ml/h into the intestine with and without atropine 1.8 mg were given on different days. CCK-like immunoreactivity (LI) in the plasma was measured by RIA during the intraintestinal oleate studies. CCK8 stimulated pancreatic enzyme secretion in a dose related fashion, an effect that was not modified by atropine. Intraintestinal oleate also stimulated pancreatic secretion and increased the CCK-LI in the plasma. Atropine significantly (p less than 0.05) decreased the pancreatic enzyme secretion before and during intraintestinal oleate, without effect on the CCK-LI levels. We conclude: (1) that the effect of exogenous CCK on pancreatic secretion of enzymes is not affected by atropine; (2) intraintestinal oleate stimulates pancreatic enzyme secretion significantly by an atropine-sensitive mechanism; (3) probably the atropine effect is a blockade of a cholinergic enteropancreatic reflex.
为了确定胆碱能反射在胰腺对食物分泌反应中的作用，我们研究了阿托品对胆囊收缩素八肽（CCK8）及肠内油酸刺激引起的淀粉酶分泌的影响。在6名健康志愿者身上进行了4项研究。在所有研究中，均通过双腔管抽吸十二指肠内容物，同时输注合成促胰液素（41 pmol/kg/h）作为背景。在不同日期，分别给予不同剂量的静脉注射CCK8或25 ml/h的0.42 M pH 9.4油酸溶液至肠内，同时给予或不给予1.8 mg阿托品。在肠内输注油酸的研究过程中，通过放射免疫分析法测定血浆中的CCK样免疫反应性（LI）。CCK8以剂量相关方式刺激胰腺酶分泌，阿托品对此作用无影响。肠内油酸也刺激胰腺分泌，并增加血浆中的CCK-LI。阿托品显著（p<0.05）降低肠内输注油酸前及输注过程中的胰腺酶分泌，但对CCK-LI水平无影响。我们得出以下结论：（1）外源性CCK对胰腺酶分泌的作用不受阿托品影响；（2）肠内油酸通过一种对阿托品敏感的机制显著刺激胰腺酶分泌；（3）阿托品的作用可能是阻断了胆碱能肠胰反射。
