Singer M V
J Physiol. 1983 Jun;339:75-85. doi: 10.1113/jphysiol.1983.sp014703.
The effect of atropine and truncal vagotomy on the latency of the secretory response of the exocrine pancreas to rapid intraduodenal injection of L-tryptophan, sodium oleate and HCl or to rapid intraportal injection of secretin or CCK octapeptide has been determined in conscious dogs with pancreatic fistulae. The intraduodenal stimulants were injected either alone or in the presence of an intravenous infusion of secretin, CCK octapeptide or a combination of both hormones. The mean latency of response to tryptophan alone (62 +/- 2 s), oleate alone (64 +/- 5 s) and HCl alone (91 +/- 3 s) was significantly longer (P less than 0.05) than the latency to intraportal secretin (28 +/- 5 s) or CCK octapeptide (45 +/- 4 s). Infusion of secretin alone or with CCK octapeptide significantly shortened the latency of response to tryptophan (38 +/- 3 s) and oleate (41 +/- 5 s), but had no effect on the latency to intraduodenal HCl (96 +/- 4 s). Atropine and truncal vagotomy both increased the latency to intraduodenal tryptophan and oleate threefold but did not affect the latency to intraduodenal HCl or intraportal secretin or CCK octapeptide. These data support the hypothesis that the stimulus to pancreatic fluid secretion evoked by intraduodenal HCl is humoral rather than neural, while the responses to intraduodenal tryptophan and oleate are mediated, at least in part, via an enteropancreatic, vago-vagal, cholinergic reflex.
在有胰瘘的清醒犬中，已测定了阿托品和迷走神经干切断术对胰腺外分泌腺对快速十二指肠内注射L-色氨酸、油酸钠和盐酸或快速门静脉内注射促胰液素或CCK八肽的分泌反应潜伏期的影响。十二指肠内刺激物单独注射，或在静脉输注促胰液素、CCK八肽或两种激素联合存在的情况下注射。单独对色氨酸（62±2秒）、单独对油酸钠（64±5秒）和单独对盐酸（91±3秒）反应的平均潜伏期明显长于门静脉内注射促胰液素（28±5秒）或CCK八肽（45±4秒）的潜伏期（P<0.05）。单独输注促胰液素或与CCK八肽一起输注可显著缩短对色氨酸（38±3秒）和油酸钠（41±5秒）反应的潜伏期，但对十二指肠内盐酸反应的潜伏期无影响（96±4秒）。阿托品和迷走神经干切断术均使十二指肠内色氨酸和油酸钠反应的潜伏期增加了两倍，但不影响十二指肠内盐酸、门静脉内促胰液素或CCK八肽反应的潜伏期。这些数据支持以下假说：十二指肠内盐酸引起的胰腺液分泌刺激是体液性的而非神经性的，而对十二指肠内色氨酸和油酸钠的反应至少部分是通过肠-胰、迷走-迷走胆碱能反射介导的。
