Chany C, Cerutti I, Mace B
Int J Cancer. 1983 Sep 15;32(3):379-83. doi: 10.1002/ijc.2910320319.
AKR mice, known to develop spontaneous leukemia in almost 100% of cases, were studied throughout their life-span. Different treatments combining a potent immune stimulator, Corynebacterium parvum (CP), with interferon (IFN) and arginine butyrate were initiated at the 15th week of life. In a preliminary series of experiments, CP (200 micrograms), IFN (20,000 units) and butyrate 50 mM) were employed in a well-defined chronological order. In controls, the mean survival time (MST) was 35.17 +/- 1.67 weeks and the final survival rates was 0/50 mice for all experiments. Only CP associated with arginine butyrate significantly augmented the MST (42.5 +/- 3.66 weeks) and final survival rate (9/35 mice). In an adjusted set of experiments, reducing the IFN concentration to 10,000 and 5,000 units and that of butyrate to 6 mM greatly improved the results. The MST was substantially increased with the combinations of CP + IFN + butyrate (41.4 +/- 1.86 weeks), CP + IFN (42.73 +/- 3.29 weeks) and butyrate + IFN (41 +/- 2.34 weeks), as well as the final survival rates (8/15, 10/15 and 6/15 mice respectively). An important finding was that when CP and IFN were used separately, they were ineffective.
已知几乎100%的AKR小鼠会自发患上白血病，对其进行了终生研究。在小鼠出生第15周时，开始采用不同的治疗方法，将强效免疫刺激剂微小棒状杆菌（CP）与干扰素（IFN）和丁酸精氨酸联合使用。在初步系列实验中，按照明确的时间顺序使用CP（200微克）、IFN（20,000单位）和丁酸（50毫摩尔）。在对照组中，所有实验的平均生存时间（MST）为35.17±1.67周，最终生存率为0/50只小鼠。只有CP与丁酸精氨酸联合使用能显著延长MST（42.5±3.66周）并提高最终生存率（9/35只小鼠）。在一组调整后的实验中，将IFN浓度降至10,000单位和5,000单位，丁酸浓度降至6毫摩尔，结果得到了极大改善。CP + IFN + 丁酸、CP + IFN以及丁酸 + IFN组合的MST均大幅增加（分别为41.4±1.86周、42.73±3.29周和41±2.34周），最终生存率也有所提高（分别为8/15只、10/15只和6/15只小鼠）。一个重要发现是，当单独使用CP和IFN时，它们没有效果。
