Yamamoto H, Bitoh S, Fujimoto S
J Exp Med. 1983 Aug 1;158(2):635-40. doi: 10.1084/jem.158.2.635.
MOPC-104E (M104E) idiotype-specific and major histocompatibility complex-restricted T lymphocyte activities were investigated in BALB/c (Igh-1a) and its Igh-1-congenic strains of mice, such as C.AL-20 (Igh-1d), BAB-14 (Igh-1b), and CB-20 (Igh-1b). Idiotype-specific T lymphocytes could be induced in BALB/c and BAB-14 by immunization of viable M104E tumor cells followed by surgical removal and in vitro restimulation with the homologous tumor cells. On the other hand, C.AL-20 and CB-20 mice did not show the idiotype-recognizing capacity even though they could mount comparable cytotoxic T lymphocyte activities against M104E to BALB/c and BAB-14. The results strongly suggest that the inducibility of M104E cross-reactive idiotypy closely paralleled the producibility of corresponding idiotype-specific T lymphocytes. Genetically defined VH gene products might act as internal images that construct idiotype network systems.
在BALB/c（Igh-1a）小鼠及其Igh-1同源基因品系小鼠，如C.AL-20（Igh-1d）、BAB-14（Igh-1b）和CB-20（Igh-1b）中，研究了MOPC-104E（M104E）独特型特异性和主要组织相容性复合体限制性T淋巴细胞活性。通过用活的M104E肿瘤细胞免疫，随后手术切除并在体外用同源肿瘤细胞再次刺激，可在BALB/c和BAB-14小鼠中诱导出独特型特异性T淋巴细胞。另一方面，C.AL-20和CB-20小鼠尽管对M104E能产生与BALB/c和BAB-14相当的细胞毒性T淋巴细胞活性，但却未表现出独特型识别能力。结果强烈表明，M104E交叉反应独特型的诱导性与相应独特型特异性T淋巴细胞的产生密切平行。基因定义的VH基因产物可能作为构建独特型网络系统的内影像发挥作用。
