Woodland R, Cantor H
Eur J Immunol. 1978 Aug;8(8):600-6. doi: 10.1002/eji.1830080812.
We have tested the proposition that induction of certain sets of B cell clones to produce antibody requires a signal from T helper cells that recognize idiotypic determinants expressed on Ig receptors of the relevant B cell clones. The approach is based on the analysis of T cell populations required to induce B cells to secrete anti-arsonate antibodies that are marked by a cross-reactive idiotype (CRId). crid+ anti-azophenyl arsonate (Ar) antibodies are produced in A/J strain mice after immunization with Ar keyhole limpet hemocyanin and represent 20--70% of the total anti-Ar antibody response. These studies indicate that antibody secretion by idiotype+ B memory cells requires two signals: one provided by "carrier"-specific Ly-1 cells, and a second delivered by idiotype-specific Ly-1 cells. Both signals are required for optimal induction of idiotype+ B memory clones.
诱导某些B细胞克隆产生抗体需要来自T辅助细胞的信号，这些T辅助细胞能够识别相关B细胞克隆的Ig受体上表达的独特型决定簇。该方法基于对诱导B细胞分泌由交叉反应独特型（CRId）标记的抗砷酸盐抗体所需的T细胞群体的分析。在用砷酸钥孔戚血蓝蛋白免疫后，A/J品系小鼠会产生CRId+抗偶氮苯砷酸盐（Ar）抗体，其占总抗Ar抗体反应的20%-70%。这些研究表明，独特型+B记忆细胞分泌抗体需要两个信号：一个由“载体”特异性Ly-1细胞提供，另一个由独特型特异性Ly-1细胞传递。这两个信号都是诱导独特型+B记忆克隆的最佳诱导所必需的。
