针对HLA的单克隆抗体可识别牛白细胞抗原（BoLA）上的单态性和多态性表位。

Monoclonal antibodies to HLA recognize monomorphic and polymorphic epitopes on BoLA.

作者信息

Chardon P, Kalil J, Leveziel H, Colombani J, Vaiman M

出版信息

Tissue Antigens. 1983 Jul;22(1):62-71. doi: 10.1111/j.1399-0039.1983.tb01167.x.

DOI:10.1111/j.1399-0039.1983.tb01167.x

PMID:6193607

Abstract

Fourteen monoclonal antibodies recognizing monomorphic and polymorphic epitopes on class I and class II antigens of the human MHC have been assayed on lymphocytes of a panel of 20-150 BoLA typed bovine animals from 12 different breeds. Some monomorphic antibodies cross-reacted and others did not. Two polymorphic monoclonal antibodies in man recognize a polymorphism in cows that follows allospecificities (BoLA-w3, w9) already described. Immunoprecipitation experiments with monomorphic anti-B2m and anti-HLA-DR monoclonal antibodies have shown that these cross-reactions concern BoLA antigens. They also revealed that Ia-like antigens in cattle present the same two chain features characterized in other species.

摘要

我们使用14种识别人类主要组织相容性复合体（MHC）I类和II类抗原上单一型和多态型表位的单克隆抗体，对来自12个不同品种、20 - 150头经牛白细胞抗原（BoLA）分型的牛的淋巴细胞进行了检测。一些单一型抗体发生了交叉反应，而另一些则没有。人类的两种多态型单克隆抗体识别出牛中一种遵循已描述的同种特异性（BoLA - w3、w9）的多态性。用单一型抗β2微球蛋白（B2m）和抗人白细胞抗原 - DR（HLA - DR）单克隆抗体进行的免疫沉淀实验表明，这些交叉反应涉及BoLA抗原。实验还揭示，牛中的I类样抗原呈现出与其他物种相同的两条链的特征。