Cross reaction of monoclonal antibodies to human MHC class I and class II products with bovine lymphocyte subpopulations.

Peripheral blood lymphocytes (PBL) from cattle have been separated into T & B cell subpopulations using a panning technique. These T and B cell preparations have then been tested by direct and indirect complement mediated cytotoxicity tests with a series of monoclonal antibodies (Moabs) reacting with HLA class I and class II products. The anti-class I monoclonals were monomorphic or non-reactive and where reactive killed both B and T cells. The anti-class II monoclonals reacted only with B lymphocytes. There was no relationship between their reactivity pattern and any BoLA specificity.