从二倍体人成纤维细胞中分离和复性α2-巨球蛋白受体
Isolation and renaturation of alpha 2-macroglobulin receptor from diploid human fibroblasts.
作者信息
Frey J, Afting E G
出版信息
Biochem J. 1983 Aug 15;214(2):629-31. doi: 10.1042/bj2140629.
Abstract
alpha 2-Macroglobulin receptor was extracted from human diploid fibroblasts and purified by affinity chromatography in a single step. The receptor had mol.wt. 125 000 after sodium dodecyl sulphate (SDS)/polyacrylamide-gel electrophoresis. The isolated receptor was separated by SDS/polyacrylamide-gel electrophoresis, transferred on to nitrocellulose sheets and subsequently renatured, as shown by a specific binding test, by incubation with Nonidet P40.
摘要
α2-巨球蛋白受体从人二倍体成纤维细胞中提取，并通过亲和色谱一步纯化。经十二烷基硫酸钠（SDS）/聚丙烯酰胺凝胶电泳后，该受体的分子量为125000。分离得到的受体经SDS/聚丙烯酰胺凝胶电泳分离，转移至硝酸纤维素膜上，随后通过与去氧胆酸钠P40孵育进行复性，特异性结合试验表明复性成功。
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