Kidd S, Lockett T J, Young M W
Cell. 1983 Sep;34(2):421-33. doi: 10.1016/0092-8674(83)90376-8.
The Notch locus appears to correspond to a 37 kb transcription unit. Homologous poly(A)+ RNA is about 11.7 kb. Nine RNA coding regions have been detected and localized within the transcription unit by S1 nuclease analyses. These range in size from 130 to 7250 bp. Sequences within the 3' coding region are variably used. Twenty-four Notch locus "point" mutations have been examined. Seven are associated with DNA insertions. Four insertions are associated with dominant mutations and are located within or very near RNA coding sequences of the 37 kb transcription unit. Three insertions are associated with recessive mutations and fall within intervening sequences. The positions of all insertions agree with the genetic map. It is estimated that, in the Notch region, one map unit corresponds to approximately 275 kb. On this basis, most of the 17 mutations that were not associated with DNA insertions can be placed within coding sequences of the 37 kb transcription unit.
Notch基因座似乎对应于一个37 kb的转录单位。同源多聚腺苷酸加尾（poly(A)+）RNA约为11.7 kb。通过S1核酸酶分析，在转录单位内检测到9个RNA编码区并确定了其位置。这些编码区大小从130到7250 bp不等。3'编码区内的序列被可变地使用。已检测了24个Notch基因座“点”突变。其中7个与DNA插入有关。4个插入与显性突变相关，位于37 kb转录单位的RNA编码序列内或非常靠近该序列。3个插入与隐性突变相关，位于间隔序列内。所有插入的位置与遗传图谱一致。据估计，在Notch区域，一个遗传图谱单位大约对应275 kb。基于此，17个与DNA插入无关的突变中的大多数可定位在37 kb转录单位的编码序列内。
