Herrmann K S
Microvasc Res. 1983 Sep;26(2):238-49. doi: 10.1016/0026-2862(83)90073-0.
Excitation of intravascular fluorescein isothiocyanate (FITC)-dextran by light induces platelet aggregation in arterioles of the hamster cheek pouch. Time to aggregate appearance (TAA) was found to be 45 +/- 5.6 sec in vessels of 10-14 micron diameter. The interindividual differences of TAA are low (coefficient of variance = 12.5%) and the TAA in individual animals can be reproduced for several hours with a coefficient of variance of 14.5%. TAA decreased at higher concentrations of intravascular FITC-dextran (r = 0.98) and with increased energy of the exciting beam. Scavengers of singlet oxygen, sodium azide, and trans-beta-carotene, delayed TAA. A scavenger of hydroxyl radicals, dimethyl sulfoxide, did not influence TAA. Acetylsalicylic acid showed a dose-dependent inhibitory effect from 1 (P less than 0.01) to 100 mg/kg body weight ia. The time-dependent increase of TAA after administration of 100 mg/kg acetylsalicylic acid is demonstrated; the dosage finally delayed TAA to longer than 360 seconds. The findings suggest that platelet formation, initiated by exciting FITC-dextran may involve local production of singlet oxygen. The consequently observed damaging process and the primary role of the endothelial cell damage are described. It is postulated that the described procedure provides a new model of intravascular platelet aggregation, characterized by a good reproducibility. A new damaging mechanism is proposed to study platelet aggregation in vivo.
血管内异硫氰酸荧光素（FITC）-葡聚糖受光激发会诱导仓鼠颊囊小动脉中的血小板聚集。在直径为10 - 14微米的血管中，聚集出现时间（TAA）为45±5.6秒。TAA的个体间差异较小（变异系数 = 12.5%），并且在个体动物中，TAA在数小时内可重复出现，变异系数为14.5%。在较高浓度的血管内FITC-葡聚糖时，TAA降低（r = 0.98），并且随着激发光束能量增加，TAA也降低。单线态氧清除剂叠氮化钠和反式β-胡萝卜素会延迟TAA。羟基自由基清除剂二甲基亚砜对TAA没有影响。乙酰水杨酸从1（P＜0.01）到100毫克/千克体重腹腔注射时显示出剂量依赖性抑制作用。在给予100毫克/千克乙酰水杨酸后，TAA随时间的增加得到了证实；最终该剂量将TAA延迟至超过360秒。这些发现表明，由激发FITC-葡聚糖引发的血小板形成可能涉及单线态氧的局部产生。描述了由此观察到的损伤过程以及内皮细胞损伤的主要作用。据推测，所描述的程序提供了一种血管内血小板聚集的新模型，其特点是具有良好的可重复性。提出了一种新的损伤机制来研究体内血小板聚集。
