Luchins J, Beychok S
Science. 1978 Jan 27;199(4327):425-6. doi: 10.1126/science.619462.
A stopped-flow circular dichroism instrument, with a total accessible wavelength range of 200 to 750 nanometers, has been constructed and provides a spectroscopic method for kinetic investigations of a wide array of fast reactions in which optical activity changes in absorbing regions are involved. An important biochemical application depends on the far-ultraviolet capability, which allows observation of the rapid alterations in backbone conformation associated with folding and unfolding reactions of proteins. Results obtained by following two such reactions at 222 nanometers represent direct monitoring by circular dichroism of rapid secondary structure changes in proteins.
已构建了一台停流圆二色性仪器，其总可及波长范围为200至750纳米，该仪器提供了一种光谱方法，用于对涉及吸收区域光学活性变化的各种快速反应进行动力学研究。一个重要的生化应用取决于远紫外能力，它能够观察与蛋白质折叠和去折叠反应相关的主链构象的快速变化。在222纳米波长下跟踪两个此类反应所获得的结果代表了通过圆二色性对蛋白质二级结构快速变化的直接监测。
