Hiramine C, Hojo K
Immunobiology. 1983 Aug;165(2):161-74. doi: 10.1016/S0171-2985(83)80057-6.
The capacity of normal (unprimed) B cells and keyhole-limpet-hemocyanin(KLH)-primed B cells to present the antigen KLH to KLH-primed T cells in the guinea pig was examined with in vitro assay of lymphocyte proliferative response. Antigen-pulsed B-lymphocyte population, extensively devoid of macrophages (M phi), induced the proliferative response of KLH-primed T lymphocytes, although less effectively than the peritoneal M phi. The antigen presentation was antigen-specific. There was no substantial difference between the magnitude of T-cell response induced by KLH-primed B-cell population and that stimulated by unprimed, normal B-cell population. The antigen-presenting capacity of the B-cell population was abrogated by pretreatment with anti-guinea pig immunoglobulin (Ig) or anti-I-region-associated (Ia) antigen antiserum and complement, whereas it was not affected with anti-guinea pig M phi antiserum and complement. From studies using strain 2 and strain 13 guinea pigs, histocompatibility requirement between antigen-pulsed B cells and antigen-reactive T cells was suggested: B lymphocytes, as well as M phi, did elicit proliferative response to specific antigen, KLH or purified protein derivative of tuberculin (PPD), in syngeneic, but not in allogeneic, T cells. These results suggest that the Ia-positive, surface-Ig-bearing guinea-pig B lymphocytes - not only KLH-primed B cells but also unprimed B cells - are capable of presenting antigen to primed T cells in a major histocompatibility complex(MHC)-restricted fashion.
采用淋巴细胞增殖反应的体外测定法，检测了正常（未致敏）B细胞和钥孔戚血蓝蛋白（KLH）致敏的B细胞将抗原KLH呈递给豚鼠体内KLH致敏T细胞的能力。抗原脉冲处理的B淋巴细胞群体，大量去除了巨噬细胞（M phi），能诱导KLH致敏T淋巴细胞的增殖反应，尽管其效率低于腹膜M phi。抗原呈递具有抗原特异性。KLH致敏的B细胞群体诱导的T细胞反应强度与未致敏的正常B细胞群体刺激的T细胞反应强度之间没有实质性差异。用抗豚鼠免疫球蛋白（Ig）或抗I区相关（Ia）抗原抗血清及补体预处理可消除B细胞群体的抗原呈递能力，而抗豚鼠M phi抗血清及补体则不影响其抗原呈递能力。通过对2系和13系豚鼠的研究，提示了抗原脉冲处理的B细胞与抗原反应性T细胞之间的组织相容性要求：B淋巴细胞以及M phi确实能在同基因而非异基因T细胞中引发对特异性抗原KLH或结核菌素纯蛋白衍生物（PPD）的增殖反应。这些结果表明，Ia阳性、表面带有Ig的豚鼠B淋巴细胞——不仅是KLH致敏的B细胞，还有未致敏的B细胞——能够以主要组织相容性复合体（MHC）限制的方式将抗原呈递给致敏T细胞。
