[苏木精和伊红染色半薄切片的改良方法]
[Modified method of staining semi-thin sections with hematoxylin and eosin].
作者信息
Migalkin N S, Ir'ianov Iu M
出版信息
Arkh Patol. 1983;45(8):88-9.
PMID:6196005
Abstract
A modified method for staining of semi-thin sections with hematoxylin and eosin after removal of epoxide resin and osmium tetroxide from them is described. To enhance staining with hematoxylin, preliminary treatment with potassium permanganate and oxalic acid solutions was used. After staining with Harris hematoxylin differentiation and simultaneous toning of the nuclei with iron chloride were performed. Subsequent post-staining consists of a mixture of alcohol- and aqueous-soluble eosins acidified with glacial acetic acid.
摘要
本文描述了一种改良方法，用于在从半薄切片上去除环氧树脂和四氧化锇后，用苏木精和伊红对其进行染色。为增强苏木精染色效果，采用了高锰酸钾和草酸溶液进行预处理。在用哈里斯苏木精染色后，进行分化并同时用氯化铁对细胞核进行调色。后续复染由用冰醋酸酸化的醇溶性和水溶性伊红混合物组成。
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[Modified method of staining semi-thin sections with hematoxylin and eosin].[苏木精和伊红染色半薄切片的改良方法]
Arkh Patol. 1983;45(8):88-9.
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