Zambonin Zallone A, Teti A, Primavera M V, Pace G
Basic Appl Histochem. 1983;27(3):191-204.
The occurrence of osteoplastic activity in adult osteocytes from the femoral cortex of laying hens previously kept on a hypocalcaemic diet was studied after return of the animals to a calcium-rich alimentation. The following techniques were used: 1) tetracycline labelling of new calcifying matrix; 2) osteoid staining with alcian blue at pH 1.9 or toluidine blue at pH 3.1; 3) electron microscopic observations; 4) autoradiographic evaluation of (3H) proline uptake by osteocytes. Quantitative evaluation of (3H) proline uptake by osteocytes. Quantitative evaluation of the preparations consistently showed indication of osteoplastic activity in at least 20% of the osteocytes with relatively higher values in the metaphysis than in the diaphysis.
对先前饲喂低钙日粮的产蛋母鸡股骨皮质中的成年骨细胞，在恢复富含钙的营养后，研究其骨形成活性的发生情况。采用了以下技术：1）用四环素标记新的钙化基质；2）在pH 1.9时用阿尔辛蓝或在pH 3.1时用甲苯胺蓝对类骨质进行染色；3）电子显微镜观察；4）通过放射自显影评估骨细胞对（3H）脯氨酸的摄取。对骨细胞摄取（3H）脯氨酸进行定量评估。对制备物的定量评估始终显示，至少20%的骨细胞有骨形成活性迹象，干骺端的值相对高于骨干。
