Munjal D, Brady P G
Am J Dig Dis. 1978 Jan;23(1):57-64. doi: 10.1007/BF01072576.
Circulating levels of carcinoembryonic antigen (CEA) and glucose phosphate isomerase (GPI) have been measured and compared in 51 subjects with gastric and colonic diseases. Levels were higher in gastric and colonic cancer patients than in normals or patients with other diseases. Elevations of both these markers were most frequent in patients with metastases. Concentrations of CEA and GPI in gastrointestinal washings were also measured. No correlation was found between total protein content and concentrations of CEA or GPI in the washings. Further characterization of the perchloric acid-soluble material from colon washings by gel filtration indicated that the CEA-like substance from colon cancer patients was higher in molecular weight than standard radiolabeled CEA and CEA from normal colon washings. When tested against anti-CEA antiserum and pure CEA from a colonic cancer metastasis, all CEA preparations showed immunological identity in gel-diffusion plates; on immunoelectrophoresis similar mobility was indicative of a similar charge.
已对51例患有胃和结肠疾病的受试者的癌胚抗原（CEA）和磷酸葡萄糖异构酶（GPI）的循环水平进行了测定和比较。胃癌和结肠癌患者的水平高于正常人和患有其他疾病的患者。这两种标志物的升高在有转移的患者中最为常见。还测量了胃肠道冲洗液中CEA和GPI的浓度。未发现冲洗液中总蛋白含量与CEA或GPI浓度之间存在相关性。通过凝胶过滤对结肠冲洗液中高氯酸可溶性物质进行的进一步表征表明，来自结肠癌患者的CEA样物质的分子量高于标准放射性标记的CEA和来自正常结肠冲洗液的CEA。当用抗CEA抗血清和来自结肠癌转移灶的纯CEA进行检测时，所有CEA制剂在凝胶扩散板上均显示出免疫同一性；在免疫电泳中，相似的迁移率表明电荷相似。
