Haresnape J M, McCahon D
J Gen Virol. 1983 Nov;64 (Pt 11):2345-55. doi: 10.1099/0022-1317-64-11-2345.
Four independent antigenic determinants in the virus capsid have been defined in radioimmunoassay tests using antisera prepared against isolated structural polypeptides VP1, 2 and 3. Only one determinant was detected on the intact particle and it was contained within VP1 (called the VP1-A determinant). Three determinants were found on the 12S subparticle, two contained within VP1 (VP1-A and VP1-B determinants) and one contained within VP2 (VP2 determinant). The fourth determinant was contained within VP3 (VP3 determinant) and could only be detected when the virus was disrupted to individual polypeptides. It is concluded that the VP1-A determinant is likely to be of epidemiological importance since (i) it was present on the intact virion, (ii) variation at this determinant was detected with heterologous field strains, (iii) it contained a determinant responsible for the induction of neutralizing antibodies and (iv) it was an antigenic component of the trypsin-sensitive region of VP1 which has been shown previously by several workers to be of critical importance in the immunogenicity of the virus.
利用针对分离出的结构多肽VP1、2和3制备的抗血清，通过放射免疫分析试验已确定病毒衣壳中有四个独立的抗原决定簇。在完整颗粒上仅检测到一个决定簇，它包含在VP1内（称为VP1 - A决定簇）。在12S亚颗粒上发现了三个决定簇，两个包含在VP1内（VP1 - A和VP1 - B决定簇），一个包含在VP2内（VP2决定簇）。第四个决定簇包含在VP3内（VP3决定簇），只有当病毒被裂解成单个多肽时才能检测到。得出的结论是，VP1 - A决定簇可能具有流行病学重要性，因为：（i）它存在于完整的病毒体上；（ii）在该决定簇处检测到与异源野外毒株的变异；（iii）它包含一个负责诱导中和抗体的决定簇；（iv）它是VP1胰蛋白酶敏感区域的一个抗原成分，此前已有几位研究人员表明该区域在病毒的免疫原性中至关重要。
