通过脑内透析对多巴胺及其代谢产物进行体内测量：右旋苯丙胺后的变化。
In vivo measurement of dopamine and its metabolites by intracerebral dialysis: changes after d-amphetamine.
作者信息
Zetterström T, Sharp T, Marsden C A, Ungerstedt U
出版信息
J Neurochem. 1983 Dec;41(6):1769-73. doi: 10.1111/j.1471-4159.1983.tb00893.x.
By using a new technique, intracerebral dialysis, in combination with high performance liquid chromatography and electrochemical detection, it was possible to recover and measure endogenous extracellular dopamine, together with its metabolites dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) from the striatum and nucleus accumbens of anaesthetized or freely moving rats. In addition, measurements of extracellular 5-hydroxyindoleacetic acid, ascorbic acid, and uric acid were made. Basal extracellular concentrations of dopamine and DOPAC in the striatum were estimated to be 5 X 10(-8) M and 5 X 10(-6) M, respectively. d-Amphetamine (2 mg/kg s.c.) increased dopamine levels in the striatum perfusates by 14-fold, whereas levels of DOPAC and HVA decreased by 77% and 66%, respectively.
通过使用一种新技术——脑内透析，并结合高效液相色谱法和电化学检测，能够从麻醉或自由活动大鼠的纹状体和伏隔核中回收并测量内源性细胞外多巴胺及其代谢产物二羟基苯乙酸（DOPAC）和高香草酸（HVA）。此外，还对细胞外5-羟吲哚乙酸、抗坏血酸和尿酸进行了测量。纹状体中多巴胺和DOPAC的基础细胞外浓度估计分别为5×10⁻⁸ M和5×10⁻⁶ M。皮下注射d-苯丙胺（2 mg/kg）使纹状体灌流液中的多巴胺水平增加了14倍，而DOPAC和HVA的水平分别下降了77%和66%。
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