Sharkey K A, Williams R G, Schultzberg M, Dockray G J
Neuroscience. 1983 Nov;10(3):861-8. doi: 10.1016/0306-4522(83)90223-3.
In rats treated neonatally with capsaicin there is, in later life, a tendency tendency towards urine retention. Since capsaicin is known to cause irreversible loss of certain primary sensory neurons, notably those containing substance P, we have studied the sensory innervation of the bladder in capsaicin-treated and control rats using retrograde tracing methods and immunohistochemistry; in addition, the motor function of the bladder was assessed in in vitro experiments, using electrical field stimulation. Five days after injection of the fluorescent tracer True Blue into the wall of the bladder, numerous labelled cells were identified in dorsal root ganglia T13, L1, L2, L6, and S1 and smaller numbers of cells were found in T12 and L3. In capsaicin-treated rats the numbers of labelled cells were reduced by over 50% in L1, L6 and S1. In control rats, 10-16% of True Blue labelled cells also contained substance P as demonstrated by indirect immunofluorescence, but in capsaicin-treated rats substance P cells were virtually absent. In in vitro studies, contractions of the detrusor muscle to electrical field stimulation, both before and after atropine, were similar in control and capsaicin-treated rats. We suggest that capsaicin causes urine retention in rats due to an impairment of sensory transmission from the bladder (that could involve substance P) and a consequent failure in the normal micturition reflexes.
在新生期用辣椒素处理的大鼠在其后期生活中有出现尿潴留的倾向。由于已知辣椒素会导致某些初级感觉神经元不可逆地丧失，特别是那些含有P物质的神经元，我们使用逆行追踪方法和免疫组织化学研究了辣椒素处理组和对照组大鼠膀胱的感觉神经支配；此外，在体外实验中，利用电场刺激评估了膀胱的运动功能。将荧光示踪剂真蓝注入膀胱壁五天后，在胸13、腰1、腰2、腰6和骶1背根神经节中发现了大量标记细胞，在胸12和腰3中发现的细胞数量较少。在辣椒素处理的大鼠中，腰1、腰6和骶1中标记细胞的数量减少了50%以上。在对照组大鼠中，通过间接免疫荧光法证实，10 - 16%的真蓝标记细胞也含有P物质，但在辣椒素处理的大鼠中，几乎不存在含P物质的细胞。在体外研究中，对照组和辣椒素处理组大鼠的逼尿肌在电场刺激下以及在阿托品处理前后的收缩情况相似。我们认为，辣椒素导致大鼠尿潴留是由于膀胱感觉传导受损（可能涉及P物质）以及随之而来的正常排尿反射失败。
