Dhami D, Mitchell B S
University of Southampton, UK.
Histochem J. 1994 Mar;26(3):262-70.
Retrograde transport studies using Fast Blue dye demonstrated that the ductus deferens, seminal vesicle, prostate and rectum, but not the urinary bladder of the male guinea pig are at least in part innervated by the anterior major pelvic ganglion. In the ductus deferens, seminal vesicle and prostate innervation is derived from ipsilateral and contralateral ganglia. In addition to retrograde studies, dye-filled neurons were analysed immunohistochemically for neuronal markers and associations with specifically identified neuronal projections. Neurons of the ganglion projecting to the ductus deferens either contained tyrosine hydroxylase alone, tyrosine hydroxylase and neuropeptide Y, neuropeptide Y alone, neuropeptide Y and vasoactive intestinal peptide, or vasoactive intestinal peptide alone. These neurons were associated with three classes of neuronal projections, substance P-, leucine-enkephalin-, and methionine-enkephalin-immunoreactive. Neurons projecting to the seminal vesicles were similar to the neurons supplying the ductus deferens, except none of the seminal vesicle-specific neurons exhibited vasoactive intestinal peptide immunoreactivity. Neurons supplying the prostate were immunoreactive for either tyrosine hydroxylase or neuropeptide Y; these neurons were infrequently associated with the three classes of varicose neuronal projections. Neurons projecting to the rectum contained neuropeptide Y and were only associated with methionine-enkephalin immunoreactive neuronal projections in one animal.
使用快蓝染料进行的逆行运输研究表明，雄性豚鼠的输精管、精囊、前列腺和直肠至少部分受盆前主神经节支配，而膀胱不受其支配。在输精管、精囊和前列腺中，神经支配来自同侧和对侧神经节。除了逆行研究外，还对充满染料的神经元进行免疫组织化学分析，以检测神经元标记物以及与特定识别的神经元投射的关联。投射到输精管的神经节神经元要么仅含有酪氨酸羟化酶，要么含有酪氨酸羟化酶和神经肽Y，要么仅含有神经肽Y，要么含有神经肽Y和血管活性肠肽，要么仅含有血管活性肠肽。这些神经元与三类神经元投射相关，即P物质、亮氨酸脑啡肽和甲硫氨酸脑啡肽免疫反应性投射。投射到精囊的神经元与供应输精管的神经元相似，但精囊特异性神经元均未表现出血管活性肠肽免疫反应性。供应前列腺的神经元对酪氨酸羟化酶或神经肽Y呈免疫反应性；这些神经元很少与三类曲张神经元投射相关。投射到直肠的神经元含有神经肽Y，并且在一只动物中仅与甲硫氨酸脑啡肽免疫反应性神经元投射相关。
