Lagunoff D, Chi E Y
J Cell Biol. 1976 Oct;71(1):182-95. doi: 10.1083/jcb.71.1.182.
In the mast cell, a well-developed array of microtubules is centered around the centrioles. Complete loss of microtubules is observed when mast cells are treated with 10(-5) M colchicine for 4 h at 37 degrees C. The loss of ultrastructurally evident microtubules is associated with a marked change in the shape of mast cells from spheroids to highly irregular, frequently elongated forms with eccentric nuclei. In colchicine-treated cells the association of nucleus, Golgi apparatus, and centrioles is also lost. Mast cells exposed to 10(-5) M colchicine for 4 h at 37 degrees C retain 80% of their capacity to release histamine when stimulated by polymyxin B. Exocytosis is evident in stimulated cells pretreated with colchicine and lacking identifiable microtubules. When the conditions of exposure of mast cells to colchicine are varied with respect to the concentration of colchicine, the length of exposure, and the temperature of exposure, dissociation between deformation of cell shape and inhibition of histamine secretion is observed. These observations indicate that microtubules are not essential for mast cell histamine release and bring into question the assumption that the inhibitory effect of colchicine on mast cell secretion depends on interference with microtubule integrity.
在肥大细胞中，一系列发育良好的微管以中心粒为中心排列。当肥大细胞在37℃下用10⁻⁵M秋水仙碱处理4小时后，可观察到微管完全丧失。超微结构上明显的微管丧失与肥大细胞形状从球体显著改变为高度不规则、常呈拉长状且核偏心有关。在秋水仙碱处理的细胞中，细胞核、高尔基体和中心粒之间的联系也丧失了。在37℃下用10⁻⁵M秋水仙碱处理4小时的肥大细胞，在受到多粘菌素B刺激时，仍保留其80%的组胺释放能力。在经秋水仙碱预处理且缺乏可识别微管的受刺激细胞中，胞吐作用明显。当肥大细胞接触秋水仙碱的条件在秋水仙碱浓度、接触时间和接触温度方面有所变化时，可观察到细胞形状变形与组胺分泌抑制之间的分离。这些观察结果表明，微管对于肥大细胞组胺释放并非必不可少，并对秋水仙碱对肥大细胞分泌的抑制作用取决于对微管完整性的干扰这一假设提出了质疑。
