Stjernquist M, Håkanson R, Leander S, Owman C, Sundler F, Uddman R
Regul Pept. 1983 Sep;7(1):67-86. doi: 10.1016/0167-0115(83)90282-3.
Immunohistochemical studies of the vas deferens and seminal vesicle of mouse, guinea-pig, and rabbit showed the presence of nerve fibres containing vasoactive intestinal polypeptide (VIP), substance P (SP), and gastrin-releasing peptide (GRP) supplying the smooth muscle layers as well as blood vessels. The nerve supply was better developed in the seminal vesicle than in the vas deferens. The motor activity of the vas deferens and seminal vesicle of the guinea-pig was studied in vitro. The vas deferens responded to transmural electrical stimulation with a twitch followed by a slow contraction. The twitch was blocked by guanethidine and tetrodotoxin, but not by atropine, propranolol, phenoxybenzamine, or fluphenazine. The slow contraction exhibited features of an alpha-receptor-mediated response. SP, physalaemin and eledoisin contracted the smooth muscle and also potentiated the twitch response to electrical nerve stimulation in a concentration-dependent manner. The SP blocking agent, (D-Pro2,D-Trp7,9)-SP, affected neither the resting tension nor the response to electrical stimulation. It is therefore suggested that the SP fibres act mainly prejunctionally. VIP, Leu-enkephalin, cholecystokinin octapeptide (CCK-8), angiotensin II, vasopressin, neurotensin, bombesin, and GRP had no effect on either the resting tension or the response to electrical nerve stimulation. The seminal vesicle responded to electrical stimulation with a contraction which was unimpaired by atropine, propranolol, phenoxybenzamine, and guanethidine, but abolished by tetrodotoxin. Hence, this contraction is mediated by a non-adrenergic, non-cholinergic neurotransmitter. Bombesin, GRP, SP, physalaemin and eledoisin contracted the smooth muscle and potentiated the response to electrical stimulation. VIP, Leu-enkephalin, CCK-8, angiotensin II, vasopressin, and neurotensin had no effect on the resting tension or on the response to transmural electrical stimulation. The SP antagonist abolished the contraction elicited by SP but did not influence the response to nerve stimulation. The results suggest that the SP and GRP nerves may have prejunctional and facilitating postjunctional effects in the seminal vesicle.
对小鼠、豚鼠和兔子的输精管及精囊进行的免疫组织化学研究表明，含有血管活性肠肽（VIP）、P物质（SP）和胃泌素释放肽（GRP）的神经纤维为平滑肌层及血管提供神经支配。精囊的神经支配比输精管发育得更好。对豚鼠的输精管和精囊的运动活性进行了体外研究。输精管对跨壁电刺激的反应先是抽搐，随后是缓慢收缩。抽搐可被胍乙啶和河豚毒素阻断，但不受阿托品、普萘洛尔、酚苄明或氟奋乃静的影响。缓慢收缩表现出α受体介导反应的特征。SP、雨蛙肽和eledoisin使平滑肌收缩，并以浓度依赖的方式增强对电神经刺激的抽搐反应。SP阻断剂（D-脯氨酸2，D-色氨酸7，9）-SP对静息张力或对电刺激的反应均无影响。因此提示SP纤维主要在神经节前起作用。VIP、亮氨酸脑啡肽、胆囊收缩素八肽（CCK-8）、血管紧张素II、血管加压素、神经降压素、蛙皮素和GRP对静息张力或对电神经刺激的反应均无影响。精囊对电刺激的反应是收缩，该反应不受阿托品、普萘洛尔、酚苄明和胍乙啶的影响，但可被河豚毒素消除。因此，这种收缩是由一种非肾上腺素能、非胆碱能神经递质介导的。蛙皮素、GRP、SP、雨蛙肽和eledoisin使平滑肌收缩并增强对电刺激的反应。VIP、亮氨酸脑啡肽、CCK-8、血管紧张素II、血管加压素和神经降压素对静息张力或对跨壁电刺激的反应均无影响。SP拮抗剂消除了SP引起的收缩，但不影响对神经刺激的反应。结果提示SP和GRP神经在精囊中可能具有神经节前和促进神经节后的作用。
