免疫荧光证据表明，在有丝分裂的、基因无活性的细胞核中不存在组蛋白H1。
Immunofluorescence evidence for the absence of histone H1 in a mitotically dividing, genetically inactive nucleus.
作者信息
Johmann C A, Gorovsky M A
出版信息
J Cell Biol. 1976 Oct;71(1):89-95. doi: 10.1083/jcb.71.1.89.
Abstract
Antibodies directed against whole histone and purified lysine-rich histone H1 extracted from isolated macronuclei of the ciliate Tetrahymena were obtained and conjugated to fluorescein isothiocyanate. The fluorescein-antibody conjugates were used to directly label Tetrahymena cells. Both macro- and micronuclei were visibly fluorescent in cells stained with anti-whole histone conjugate. However, the anti-H1 conjugate only labeled macronuclei. This in situ demonstration of the lack of positive immunofluorescent staining of micronuclei with anti-H1 conjugate provide further evidence for the absence of H1 in the genetically inactive, mitotically dividing Tetrahymena micronucleus.
摘要
制备了针对从纤毛虫四膜虫分离的大核中提取的全组蛋白和纯化的富含赖氨酸的组蛋白H1的抗体，并将其与异硫氰酸荧光素偶联。荧光素-抗体偶联物用于直接标记四膜虫细胞。在用抗全组蛋白偶联物染色的细胞中，大核和小核均可见荧光。然而，抗H1偶联物仅标记大核。抗H1偶联物对小核缺乏阳性免疫荧光染色的原位证明，为在遗传上无活性、进行有丝分裂的四膜虫小核中不存在H1提供了进一步的证据。
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