在有丝分裂的、基因无活性的细胞核中缺乏组蛋白F1。
Absence of histone F1 in a mitotically dividing, genetically inactive nucleus.
作者信息
Gorovsky M A, Keevert J B
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2672-6. doi: 10.1073/pnas.72.7.2672.
Abstract
Histones were extracted from macro- and micronuclear chromatin of the ciliated protozoan Tetrahymena pyriformis. Conditions that resulted in macronuclear chromatin containing large amounts of histone F1 yielded micronuclear chromatin in which this histone was absent. Evidence is presented indicating that the absence of F1 from micronuclei is not a preparative artifact and that histone F1 is replaced by other histone fractions. Since micronuclei divide mitotically, while macronuclei divide amitotically, these results suggest that histone F1 and its phosphorylation do not play an indispensable role in the process of mitotic chromosome condensation, in chromosome replication, or in the separation of newly synthesized chromatids.
摘要
从纤毛原生动物梨形四膜虫的大核和小核染色质中提取组蛋白。导致含有大量组蛋白F1的大核染色质产生的条件，会使小核染色质中不存在这种组蛋白。有证据表明，小核中不存在F1并非制备过程中的假象，且组蛋白F1被其他组蛋白组分所取代。由于小核进行有丝分裂，而大核进行无丝分裂，这些结果表明组蛋白F1及其磷酸化在有丝分裂染色体浓缩过程、染色体复制过程或新合成染色单体的分离过程中并不起不可或缺的作用。
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