Schulte B A, Spicer S S
Am J Anat. 1983 Nov;168(3):345-62. doi: 10.1002/aja.1001680308.
Paraffin sections of mouse and rat kidney were stained with a battery of ten lectin-horseradish peroxidase conjugates and lectin binding was correlated with the ultrastructural distribution of periodate-reactive sugar residues as determined by the periodic acid-thiocarbohydrazide-silver proteinate technique. Various segments of the uriniferous tubule in both species showed differential affinity for labelled lectins. Significant differences were also evident between comparable tubular segments in mouse and rat kidneys. Neutral glycoconjugates containing terminal beta-galactose and terminal alpha-N-acetylgalactosamine were prevalent on the luminal surface of the proximal convoluted tubule in the rat, but alpha-N-acetylgalactosamine was absent in this site in the mouse. In both species, terminal N-acetylglucosamine was abundant in the brush border of proximal straight tubules but absent in proximal convolutions. Fucose was demonstrated in both proximal and distal segments of mouse kidney tubules but only in the distal nephron and collecting ducts in the rat. Lectin staining revealed striking heterogeneity in the structure and distribution of cellular glycoconjugates. Such cellular heterogeneity was previously unrecognizable with earlier histochemical methods. The marked cellular heterogeneity observed with several lectin-conjugates in distal convoluted tubules and collecting ducts of both species raises a prospect that lectins can provide specific markers for intercalated and principal cells in the mammalian kidney. Glycoconjugates containing terminal sialic acid and penultimate beta-galactose were present on vascular endothelium in both rodent kidneys, as were terminal alpha-galactose residues; but both species lacked reactivity for Ulex europeus I lectin in contrast to human vascular endothelial cells. The constant binding pattern of lectin conjugates allows convenient and precise differentiation of renal tubular segments and should prove valuable in the study of changes in kidney morphology promoted by experimental manipulation or pathologic changes.
用一组十种凝集素 - 辣根过氧化物酶结合物对小鼠和大鼠肾脏的石蜡切片进行染色，并将凝集素结合与通过高碘酸 - 硫代碳酰肼 - 银蛋白技术测定的高碘酸盐反应性糖残基的超微结构分布相关联。两种物种的泌尿小管的各个节段对标记的凝集素表现出不同的亲和力。小鼠和大鼠肾脏中可比的肾小管节段之间也存在明显差异。含有末端β - 半乳糖和末端α - N - 乙酰半乳糖胺的中性糖缀合物在大鼠近端曲管的腔表面普遍存在，但在小鼠的该部位不存在α - N - 乙酰半乳糖胺。在两种物种中，末端N - 乙酰葡糖胺在近端直小管的刷状缘中丰富，但在近端曲管中不存在。岩藻糖在小鼠肾小管的近端和远端节段中均有显示，但在大鼠中仅在远端肾单位和集合管中存在。凝集素染色揭示了细胞糖缀合物的结构和分布存在显著异质性。这种细胞异质性以前用早期的组织化学方法无法识别。在两种物种的远端曲管和集合管中用几种凝集素结合物观察到的明显细胞异质性提出了一种前景，即凝集素可以为哺乳动物肾脏中的闰细胞和主细胞提供特异性标记。含有末端唾液酸和倒数第二个β - 半乳糖的糖缀合物在两种啮齿动物肾脏的血管内皮上均存在，末端α - 半乳糖残基也是如此；但与人类血管内皮细胞相比，两种物种对欧洲荆豆I凝集素均无反应性。凝集素结合物的恒定结合模式便于并精确区分肾小管节段，并且在研究实验操作或病理变化引起的肾脏形态变化方面应该证明是有价值的。
