Schulte B A, Spicer S S
J Histochem Cytochem. 1983 Mar;31(3):391-403. doi: 10.1177/31.3.6186733.
Lectins conjugated to horseradish peroxidase were used to stain paraffin sections of mouse, rat, and human respiratory tract tissues. An additional method was applied utilizing galactose oxidase to oxidize the C-6 hydroxyl of galactose and N-acetylgalactosamine residues and the resulting aldehyde was visualized with 2% Schiff's reagent. Sections were stained prior to and after removal of sialic acid residues. Oligosaccharides with terminal beta-galactose and alpha-N-acetylgalactosamine residues were found in all serous cells in the mouse trachea but were never seen in human tracheal serous cells. About 5-10% of serous cells in the rat trachea contained terminal beta-galactose, whereas all human tracheal serous cells and the remainder of those in the rat contained oligosaccharides with terminal sialic acid and penultimate beta-galactose residues. Fucose was not detected in tracheal serous cells of any species. Mucous cells in the mouse and rat trachea produced heterogeneous oligosaccharides containing terminal alpha-N-acetylgalactosamine and/or terminal sialic acid residues in various proportions. The structure of oligosaccharides in human tracheal mucous cells varied between individuals and was related to ABO blood group reactivity. The majority of oligosaccharides in type A individuals contained terminal alpha-N-acetylgalactosamine, whereas type AB individuals had approximately equal amounts of terminal alpha-galactose and alpha-N-acetylgalactosamine residues. Mucous cells in the two type O specimens examined contained a large amount of terminal beta-galactose and surprisingly, terminal fucose was not detected in these individuals. These results support biochemical studies showing structural diversity in oligosaccharide chains of respiratory tract secretions and reveal differences in glycoprotein secretion of different cell types.
将辣根过氧化物酶偶联的凝集素用于对小鼠、大鼠和人类呼吸道组织的石蜡切片进行染色。还应用了另一种方法，即利用半乳糖氧化酶氧化半乳糖和N-乙酰半乳糖胺残基的C-6羟基，并用2%的席夫试剂观察产生的醛。在去除唾液酸残基之前和之后对切片进行染色。在小鼠气管的所有浆液细胞中都发现了带有末端β-半乳糖和α-N-乙酰半乳糖胺残基的寡糖，但在人类气管浆液细胞中从未见过。大鼠气管中约5-10%的浆液细胞含有末端β-半乳糖，而所有人类气管浆液细胞以及大鼠中其余的浆液细胞都含有带有末端唾液酸和倒数第二个β-半乳糖残基的寡糖。在任何物种的气管浆液细胞中都未检测到岩藻糖。小鼠和大鼠气管中的黏液细胞产生了含有不同比例的末端α-N-乙酰半乳糖胺和/或末端唾液酸残基的异质寡糖。人类气管黏液细胞中寡糖的结构因人而异，并且与ABO血型反应性有关。A型个体中的大多数寡糖含有末端α-N-乙酰半乳糖胺，而AB型个体中末端α-半乳糖和α-N-乙酰半乳糖胺残基的含量大致相等。在检查的两个O型标本中，黏液细胞含有大量的末端β-半乳糖，令人惊讶的是，在这些个体中未检测到末端岩藻糖。这些结果支持了生化研究，表明呼吸道分泌物寡糖链存在结构多样性，并揭示了不同细胞类型糖蛋白分泌的差异。
