Nugent L J, Jain R K
Cancer Res. 1984 Jan;44(1):238-44.
Extravascular transport of fluorescein isothiocyanate-conjugated bovine serum albumin and a graded series of fluorescein isothiocyanate-dextrans from Mr 19,400 to 71,800 were studied in both normal tissue (granulation) and tumor (VX2 carcinoma) grown in a rabbit ear chamber. Sodium fluorescein was used as a representative small molecule. A one-dimensional diffusion model adequately described extravascular transport in both normal and tumor tissue. Measured diffusion coefficients showed a relationship with molecular size which progressively deviates from that of free diffusion in water, with values for albumin being significantly reduced from that for a dextran of equivalent size. Macromolecular transport in tumor tissue was hindered to a lesser extent than in normal tissue, which is consistent with reports of reduced contents of glycosaminoglycans, and markedly large interstitial space in tumors. Diffusion coefficients for dextran were found to vary with molecular weight according to the expression, D = a(Mr)b, in both normal tissue (a = 10(6) and b = -2.96) and tumor (a = 2.51 X 10(-2) and b = 1.14).
在兔耳腔中生长的正常组织（肉芽组织）和肿瘤（VX2癌）中，研究了异硫氰酸荧光素偶联的牛血清白蛋白以及分子量从19,400至71,800的一系列分级异硫氰酸荧光素葡聚糖的血管外转运。使用荧光素钠作为代表性小分子。一维扩散模型充分描述了正常组织和肿瘤组织中的血管外转运。测得的扩散系数显示出与分子大小的关系，该关系逐渐偏离水中自由扩散的关系，白蛋白的值比同等大小的葡聚糖的值显著降低。肿瘤组织中的大分子转运比正常组织受到的阻碍较小，这与关于肿瘤中糖胺聚糖含量降低和间隙空间明显增大的报道一致。发现葡聚糖的扩散系数在正常组织（a = 10(6)且b = -2.96）和肿瘤（a = 2.51×10(-2)且b = 1.14）中均根据表达式D = a(Mr)b随分子量而变化。
