Cheeseman H J, Pinnock R D, Henderson G
Eur J Pharmacol. 1983 Oct 14;94(1-2):93-9. doi: 10.1016/0014-2999(83)90445-4.
We have made extracellular recordings from locus coeruleus neurones contained in a pontine slice preparation. The in vitro technique permits application of known concentrations of drugs in the perfusion medium. Substance P excites locus coeruleus neurones in a concentration-dependent manner. The activity of substance P was found to reside in the C terminal region of the molecule. The relative potency of substance P and related peptides suggests that the substance P receptors present within the locus coeruleus are predominately of the substance P-P type. The synthetic analogue [D-Pro2,D-Trp7.9]substance P was not found to antagonise the action of substance P on locus coeruleus neurones.
我们对脑桥切片制备中含有的蓝斑神经元进行了细胞外记录。体外技术允许在灌流介质中应用已知浓度的药物。P物质以浓度依赖的方式兴奋蓝斑神经元。发现P物质的活性存在于该分子的C末端区域。P物质及相关肽的相对效价表明，蓝斑内存在的P物质受体主要是P物质-P型。未发现合成类似物[D-脯氨酸2，D-色氨酸7,9]P物质能拮抗P物质对蓝斑神经元的作用。
