利用抗Ia单克隆抗体确定兔Ia分子在免疫功能中的作用。
The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody.
作者信息
Lobel S A, Knight K L
出版信息
Immunology. 1984 Jan;51(1):35-43.
We have produced a mouse anti-rabbit Ia monoclonal antibody (MAb) that detects an isotypic determinant on all rabbit Ia molecules. This MAb precipitates three polypeptide chains with molecular weights of 28,000, 31,000 and 35,000, corresponding to the Ia beta, Ii and alpha chains, respectively. The anti-Ia MAb inhibits the mixed lymphocyte culture by 80%. In secondary in vitro immune response cultures, the anti-Ia MAb inhibits the proliferative response to bovine insulin and poly (Glu50Tyr50). In studies on mitogenesis it was found that the anti-Ia MAb inhibited the response to LPS but not to concanavalin A or phytohaemagglutinin. The effect of the anti-Ia MAb on other mitogens was found to vary from rabbit to rabbit.
我们制备了一种小鼠抗兔Ia单克隆抗体（MAb），它能检测所有兔Ia分子上的一个同种型决定簇。这种单克隆抗体沉淀出三条分子量分别为28,000、31,000和35,000的多肽链，分别对应于Iaβ、Ii和α链。抗Ia单克隆抗体可使混合淋巴细胞培养受到80%的抑制。在二次体外免疫应答培养中，抗Ia单克隆抗体抑制对牛胰岛素和聚（谷氨酸50酪氨酸50）的增殖反应。在有丝分裂原研究中发现，抗Ia单克隆抗体抑制对脂多糖的反应，但不抑制对刀豆球蛋白A或植物血凝素的反应。发现抗Ia单克隆抗体对其他有丝分裂原的作用因兔而异。
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