Engelhard V H, Powers G A, Moore L C, Holterman M J, Correa-Freire M C
J Immunol. 1984 Jan;132(1):76-80.
HLA-A2 and -B7 antigens were introduced into EL4 (H-2b) cells by cell-liposome fusion and were used as targets or stimulators for cytotoxic T lymphocytes (CTL) generated in C57B1/6 (H-2b) mice. It was found that such EL4-HLA cells were not recognized by CTL that had been raised against either a human cell line bearing these HLA antigens or the purified HLA-A2 and -B7 antigens reconstituted into liposomes. In addition, EL4-HLA cells were not capable of inducing CTL that could recognize a human cell line bearing HLA-A2 and -B7 antigens. Instead, EL4-HLA cells induced CTL that specifically lysed EL4-HLA cells and not human cells expressing HLA-A2 and -B7. CTL recognition required the presence of HLA antigens on the EL4 cell surface and was inhibited by antibodies against either H-2b or HLA-A/B. Monoclonal antibody binding studies showed that the expected polymorphic determinants of the HLA-A2 and -B7 antigens were still present on EL4-HLA cells. However, the specificity of CTL or their precursors that are capable of recognizing HLA-A2 or -B7 was altered after these antigens became associated with the EL4 surface. Possible explanations for these results are discussed.
通过细胞 - 脂质体融合将HLA - A2和 - B7抗原导入EL4（H - 2b）细胞，并将其用作C57B1/6（H - 2b）小鼠中产生的细胞毒性T淋巴细胞（CTL）的靶标或刺激物。结果发现，针对携带这些HLA抗原的人细胞系或重构成脂质体的纯化HLA - A2和 - B7抗原产生的CTL不能识别此类EL4 - HLA细胞。此外，EL4 - HLA细胞不能诱导能够识别携带HLA - A2和 - B7抗原的人细胞系的CTL。相反，EL4 - HLA细胞诱导的CTL特异性裂解EL4 - HLA细胞，而不裂解表达HLA - A2和 - B7的人细胞。CTL识别需要EL4细胞表面存在HLA抗原，并受到抗H - 2b或HLA - A/B抗体的抑制。单克隆抗体结合研究表明，HLA - A2和 - B7抗原预期的多态性决定簇仍存在于EL4 - HLA细胞上。然而，这些抗原与EL4表面结合后，能够识别HLA - A2或 - B7的CTL或其前体的特异性发生了改变。文中讨论了这些结果的可能解释。
