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使用伯乐银染法鉴定小核核糖核蛋白抗原的凝胶纯化RNA成分。

Use of the Bio-Rad silver stain to identify gel purified RNA components of small nuclear ribonucleoprotein antigens.

作者信息

McNeilage L J, Whittingham S

出版信息

J Immunol Methods. 1984 Feb 10;66(2):253-60. doi: 10.1016/0022-1759(84)90336-3.

DOI:10.1016/0022-1759(84)90336-3
PMID:6197482
Abstract

The ultrasensitive silver staining procedure developed for the detection of proteins in polyacrylamide gels has been used to identify the RNA component of small nuclear ribonucleoprotein antigens recognised by antinuclear antibodies. The technique described is a simple, safe and inexpensive procedure with a sensitivity comparable with that obtained by biosynthetic labelling of RNA with 32P.

摘要

为检测聚丙烯酰胺凝胶中的蛋白质而开发的超灵敏银染程序，已被用于鉴定抗核抗体识别的小核核糖核蛋白抗原的RNA成分。所描述的技术是一种简单、安全且廉价的程序，其灵敏度与用32P对RNA进行生物合成标记所获得的灵敏度相当。

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