Sibbitt W L, Bankhurst A D, Jumonville A J, Saiki J H, Saiers J H, Doberneck R C
Cancer Res. 1984 Feb;44(2):852-6.
The natural killer (NK) activity of peripheral blood mononuclear cells from 25 patients with squamous cell carcinoma of the lung, malignant melanoma, or epitheloid cancers of the gastrointestinal tract was measured by the lysis of 51Cr-labeled K562 target cells. NK activities of many patients with lung cancer or malignant melanoma were decreased relative to normal controls. This abnormality was significantly correlated with advancing stage of disease and the percentage of monocytes in the cell suspensions. Addition of indomethacin or removal of monocytes did not restore depressed NK function to normal levels. Abnormalities of NK function did not appear to be secondary to the presence of mononuclear suppressor cells. The response to interferon was also impaired in patients with advanced disease. The number of effector:target conjugates was normal even in patients with depressed NK function; however, the number of active lytic effectors was decreased. These results imply that the cells which bind tumor targets are present in patients with advanced cancers, but these cells are either immature or functionally inactive.
通过对51Cr标记的K562靶细胞的裂解，检测了25例肺癌鳞状细胞癌、恶性黑色素瘤或胃肠道上皮样癌患者外周血单个核细胞的自然杀伤（NK）活性。许多肺癌或恶性黑色素瘤患者的NK活性相对于正常对照有所降低。这种异常与疾病的进展阶段和细胞悬液中单核细胞的百分比显著相关。添加吲哚美辛或去除单核细胞并不能使降低的NK功能恢复到正常水平。NK功能异常似乎不是单核抑制细胞存在的继发结果。晚期疾病患者对干扰素的反应也受损。即使在NK功能降低的患者中，效应细胞与靶细胞结合物的数量也是正常的；然而，活跃的裂解效应细胞的数量减少了。这些结果表明，晚期癌症患者体内存在与肿瘤靶标结合的细胞，但这些细胞要么不成熟，要么功能不活跃。
