一种用于人网织红细胞流式细胞术分析的快速活体染色程序。

A rapid, vital staining procedure for flow cytometric analysis of human reticulocytes.

作者信息

Sage B H, O'Connell J P, Mercolino T J

出版信息

Cytometry. 1983 Nov;4(3):222-7. doi: 10.1002/cyto.990040306.

PMID:6198129

Abstract

A fluorescent staining procedure based upon thioflavin T is described. This dye permits rapid, vital, nonprecipitative staining of human reticulocytes. Flow cytometric analysis of thioflavin T stained whole blood specimens results in reticulocyte counts that correlate well with new methylene blue reticulocyte counts and give coefficients of variation approaching the theoretical limit. The shape of the fluorescence histogram is qualitatively similar to the reticulocyte age profile. Paired new methylene blue and thioflavin T reticulocyte counts are presented with illustrative cases of reticulocyte maturity distributions.

摘要

本文描述了一种基于硫黄素T的荧光染色方法。这种染料可对人网织红细胞进行快速、活体、非沉淀性染色。对硫黄素T染色的全血标本进行流式细胞术分析，所得网织红细胞计数与新亚甲蓝网织红细胞计数相关性良好，变异系数接近理论极限。荧光直方图的形状在质量上与网织红细胞年龄分布相似。文中给出了配对的新亚甲蓝和硫黄素T网织红细胞计数，并配有网织红细胞成熟度分布的示例病例。