Jacobberger J W, Horan P K, Hare J D
Cytometry. 1984 Nov;5(6):589-600. doi: 10.1002/cyto.990050607.
The fluorescent dye 3,3'-dimethyloxacarbocyanine (DiOC1[3]) is taken up by all cells in mammalian blood which then fluoresce as follows: mature erythrocytes less than immature erythrocytes congruent to platelets less than leukocytes. A continuous fluorescence distribution can be generated for the red blood cells by flow cytometry and deconvolved into two arbitrary populations, mature and immature erythrocytes (mRBC and imRBC). This analysis mimics the established method of counting imRBC stained with the supravital dyes, new methylene blue, brilliant cresyl blue (BCB), and acridine orange (AO). However, the population of imRBC as quantified by DiOC1[3] fluorescence is a subset of reticulocytes (reticulocytes as determined by BCB assay). The advantages and disadvantages of using DiOC1[3], AO, or pyronine Y as reticulocyte stains are discussed.
荧光染料3,3'-二甲基氧杂羰花青（DiOC1[3]）可被哺乳动物血液中的所有细胞摄取，这些细胞随后会发出如下荧光：成熟红细胞的荧光强度低于未成熟红细胞，未成熟红细胞的荧光强度与血小板相当，血小板的荧光强度低于白细胞。通过流式细胞术可以为红细胞生成连续的荧光分布，并将其解卷积为两个任意群体，即成熟红细胞和未成熟红细胞（mRBC和imRBC）。这种分析模仿了用活体染料、新亚甲蓝、灿烂甲酚蓝（BCB）和吖啶橙（AO）染色来计数未成熟红细胞的既定方法。然而，通过DiOC1[3]荧光定量的未成熟红细胞群体是网织红细胞的一个子集（网织红细胞由BCB测定法确定）。本文讨论了使用DiOC1[3]、AO或派洛宁Y作为网织红细胞染色剂的优缺点。
