Krohne G, Debus E, Osborn M, Weber K, Franke W W
Exp Cell Res. 1984 Jan;150(1):47-59. doi: 10.1016/0014-4827(84)90700-6.
Immunofluorescence microscopy shows that the monoclonal murine antibody PKB8 stains the nuclear lamina of various somatic cells from vertebrates as diverse as mammals, birds and amphibia. It also decorates the nuclear periphery of oocytes from rat and chicken but does not react with spermatocytes, spermatids and spermatozoa. Immunoblotting experiments demonstrate reaction with lamina polypeptides A, B and C of rat, with lamina polypeptide A of chicken, and with lamina polypeptides LI and LII of erythrocytes of the frog, Xenopus laevis. Antibody PKB8 does, however, not bind, on blotted polypeptides and on sections through ovaries, to the pore complex-lamina polypeptide of Mr 68000 present in Xenopus oocytes. These results reveal the existence of a common antigenic determinant in all three lamina polypeptides of mammals, in one lamina polypeptide of chicken and in two amphibian lamina polypeptides. The immunological data also indicate that, in Xenopus laevis, pore complex-lamina polypeptides of somatic cells and oocytes are distinct. The Mr 68000 protein of Xenopus oocytes is also different from polypeptides LI and LII of somatic Xenopus cells by tryptic peptide mapping. The results suggest that nuclear pore complex-lamina polypeptides represent a family of related polypeptides containing regions highly conserved during evolution and that these polypeptides can be differentially expressed in cells of at least one species, Xenopus laevis.
免疫荧光显微镜检查显示，单克隆鼠抗体PKB8可对来自哺乳动物、鸟类和两栖动物等多种脊椎动物的各种体细胞的核纤层进行染色。它还能修饰大鼠和鸡卵母细胞的核周边，但不与精母细胞、精子细胞和精子发生反应。免疫印迹实验表明，该抗体可与大鼠的核纤层多肽A、B和C、鸡的核纤层多肽A以及非洲爪蟾红细胞的核纤层多肽LI和LII发生反应。然而，抗体PKB8在印迹多肽和卵巢切片上不与非洲爪蟾卵母细胞中存在的分子量为68000的孔复合体-核纤层多肽结合。这些结果揭示了哺乳动物的所有三种核纤层多肽、鸡的一种核纤层多肽以及两种两栖动物核纤层多肽中存在共同的抗原决定簇。免疫学数据还表明，在非洲爪蟾中，体细胞和卵母细胞的孔复合体-核纤层多肽是不同的。通过胰蛋白酶肽图谱分析，非洲爪蟾卵母细胞的分子量为68000的蛋白质也与非洲爪蟾体细胞的多肽LI和LII不同。结果表明，核孔复合体-核纤层多肽代表了一类相关多肽家族，其含有在进化过程中高度保守的区域，并且这些多肽在至少一个物种非洲爪蟾的细胞中可差异表达。
