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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Shay C E, Foster P G, Neelin J M

Department of Biology, Carleton University, Ottawa, Canada.

Comp Biochem Physiol B. 1988;91(1):69-78. doi: 10.1016/0305-0491(88)90115-0.

The relationship between sequence homology and immunological cross-reaction was investigated by enzyme-linked immunosorbent assay and immunoblotting using polyclonal antisera against lysine-rich histones (LRH) of known sequence, chicken H1 and H5, trout Hl and Xenopus H1s. 2. The order of immunological relatedness was consistent with known homologies among these LRH and goose H5, but quantitative correlations reflected varied locations of antigenic determinants. 3. When immunoblotting was extended to LRH from eight more vertebrates, it was evident that avian H5, mammalian H1o and anuran H1s form a sub-class, to which turtle H1s may belong, that urodele erythrocytes contain no H5-like histone and that fish "H5" is more like H1 than the H5/H1s/H1o subclass.

通过酶联免疫吸附测定法和免疫印迹法，使用针对已知序列的富含赖氨酸组蛋白（LRH）、鸡H1和H5、鳟鱼H1以及非洲爪蟾H1的多克隆抗血清，研究了序列同源性与免疫交叉反应之间的关系。2. 免疫相关性顺序与这些LRH和鹅H5之间已知的同源性一致，但定量相关性反映了抗原决定簇的不同位置。3. 当免疫印迹法扩展到另外八种脊椎动物的LRH时，很明显禽类H5、哺乳动物H1o和无尾目动物H1形成一个亚类，龟类H1可能属于该亚类，有尾目动物红细胞不含H5样组蛋白，并且鱼类“ H5”比H5 / H1s / H1o亚类更像H1。

Shay C E, Foster P G, Neelin J M

Department of Biology, Carleton University, Ottawa, Canada.

Comp Biochem Physiol B. 1988;91(1):69-78. doi: 10.1016/0305-0491(88)90115-0.

The relationship between sequence homology and immunological cross-reaction was investigated by enzyme-linked immunosorbent assay and immunoblotting using polyclonal antisera against lysine-rich histones (LRH) of known sequence, chicken H1 and H5, trout Hl and Xenopus H1s. 2. The order of immunological relatedness was consistent with known homologies among these LRH and goose H5, but quantitative correlations reflected varied locations of antigenic determinants. 3. When immunoblotting was extended to LRH from eight more vertebrates, it was evident that avian H5, mammalian H1o and anuran H1s form a sub-class, to which turtle H1s may belong, that urodele erythrocytes contain no H5-like histone and that fish "H5" is more like H1 than the H5/H1s/H1o subclass.

通过酶联免疫吸附测定法和免疫印迹法，使用针对已知序列的富含赖氨酸组蛋白（LRH）、鸡H1和H5、鳟鱼H1以及非洲爪蟾H1的多克隆抗血清，研究了序列同源性与免疫交叉反应之间的关系。2. 免疫相关性顺序与这些LRH和鹅H5之间已知的同源性一致，但定量相关性反映了抗原决定簇的不同位置。3. 当免疫印迹法扩展到另外八种脊椎动物的LRH时，很明显禽类H5、哺乳动物H1o和无尾目动物H1形成一个亚类，龟类H1可能属于该亚类，有尾目动物红细胞不含H5样组蛋白，并且鱼类“ H5”比H5 / H1s / H1o亚类更像H1。