Riley D A, Lang D H
J Hand Surg Am. 1984 Jan;9A(1):112-20. doi: 10.1016/s0363-5023(84)80198-7.
The return of usable function after injury of peripheral nerves depends upon the appropriate regeneration of axons to their end organs. Debridement trimmings of severed nerves harvested during surgery were stained to demonstrate carbonic anhydrase activity. This histochemical method can be accomplished within 3 to 4 hours of receiving the tissue. Nerve fascicles were readily discriminated from one another by the individual staining patterns of their constituent axons. Axoplasmic staining was predominantly a feature of sensory fibers, and myelin staining was characteristic of skeletal motor axons. Carbonic anhydrase histochemistry may provide a means of accurately matching fascicles in cut nerve ends.
周围神经损伤后功能的恢复取决于轴突能否成功再生至其终末器官。手术中采集的离断神经的清创修剪组织被染色以显示碳酸酐酶活性。这种组织化学方法可在收到组织后的3至4小时内完成。通过其组成轴突的个体染色模式，神经束很容易相互区分。轴浆染色主要是感觉纤维的特征，而髓鞘染色是骨骼肌运动轴突的特征。碳酸酐酶组织化学可为准确匹配切断神经断端的神经束提供一种方法。
