Tanabe K
J Protozool. 1983 Nov;30(4):707-10. doi: 10.1111/j.1550-7408.1983.tb05347.x.
The cationic permeant fluorescent dye rhodamine 123 (R123) was used to stain Plasmodium yoelii-infected mouse erythrocytes. Fluorescence microscopic observations demonstrated that the parasite, but not the matrix of the infected erythrocyte, accumulated the dye. Differences in fluorescence intensity could not be found at the various developmental stages of the parasite; however, quantitation of the cell-associated dye revealed an increase in R123 uptake with parasite development. The retention of the parasite-associated dye, as measured by fluorescence microscopy and spectrophotometry after extraction of R123 with butanol, was markedly reduced by treatment of the infected erythrocytes with a proton ionophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and an inhibitor of proton ATPase, dicyclohexylcarbodiimide (DCCD). These results indicate that the accumulation and retention of R123 in P. yoelii reflect the parasite membrane potential and suggest that the parasite plasma membrane has a membrane potential-generating proton pump.
阳离子渗透性荧光染料罗丹明123（R123）用于对约氏疟原虫感染的小鼠红细胞进行染色。荧光显微镜观察表明，染料在疟原虫中积累，而不在被感染红细胞的基质中积累。在疟原虫的不同发育阶段未发现荧光强度差异；然而，对细胞相关染料的定量分析显示，随着疟原虫的发育，R123摄取量增加。在用丁醇提取R123后，通过荧光显微镜和分光光度法测量，疟原虫相关染料的保留量在使用质子离子载体羰基氰化物间氯苯腙（CCCP）和质子ATP酶抑制剂二环己基碳二亚胺（DCCD）处理感染的红细胞后显著降低。这些结果表明，R123在约氏疟原虫中的积累和保留反映了疟原虫膜电位，并提示疟原虫质膜具有产生膜电位的质子泵。
