Rimm I J, Bhan A K, Schneeberger E E, Schlossman S F, Reinherz E L
Clin Immunol Immunopathol. 1984 Apr;31(1):56-64. doi: 10.1016/0090-1229(84)90189-2.
Thymic epithelial cells have a critical influence on T-cell differentiation. In order to characterize these cells in humans, a serum-free growth medium was developed for their long-term culture. Important components of this medium included transferrin, epidermal growth factor, prostaglandin E1, and selenious acid. The presence of a keratin cytoskeleton, tonofilaments, and desmosomes confirmed the epithelial nature of these cells. Indirect immunofluorescence study of these epithelial cells demonstrated the presence of Ia and B-2 microglobulin antigens. The availability of highly enriched thymic epithelial cultures should simplify the functional characterization of this cell.
胸腺上皮细胞对T细胞分化具有关键影响。为了在人类中对这些细胞进行表征，开发了一种无血清生长培养基用于其长期培养。该培养基的重要成分包括转铁蛋白、表皮生长因子、前列腺素E1和亚硒酸。角蛋白细胞骨架、张力丝和桥粒的存在证实了这些细胞的上皮性质。对这些上皮细胞的间接免疫荧光研究表明存在Ia和B-2微球蛋白抗原。高度富集的胸腺上皮培养物的可得性应能简化对该细胞的功能表征。
