Pawelec G, Wernet P, Rosenlund R, Blaurock M, Schneider E M
Hum Immunol. 1984 Mar;9(3):145-57. doi: 10.1016/0198-8859(84)90042-9.
From a total of 37 different priming combinations between donors matched for HLA-A,B, and/or Dw/DR, but mismatched for SB, antigens, T cell clones strongly restimulated with concordance for SB specificities were isolated from only two. Most of the alloproliferative (PLT) clones obtained were restimulated by determinants not correlated with any currently known HLA product. Nonetheless, their stimulation was inhibited by a monoclonal antibody TU 39, which preferentially blocks stimulation by SB-, rather than by Dw/DR-associated determinants. Despite having an OKT4+, OKT-, Leu8- phenotype, and secreting Interleukin-2 after contact with stimulatory cells, these clones strongly suppressed proliferative responses of cloned PLT reagents as well as unprimed lymphocytes in mixed leukocyte cultures. They may thus represent a novel type of immunoregulatory T cell, stimulated by SB-related antigens, which despite their "helper/inducer" phenotype are able directly to suppress lymphoproliferative responses.
在37种不同的启动组合中，供体之间HLA - A、B和/或Dw/DR相匹配，但SB抗原不匹配，仅从其中两个组合中分离出了与SB特异性一致且受到强烈再刺激的T细胞克隆。所获得的大多数同种异体增殖性（PLT）克隆是由与目前已知的任何HLA产物均不相关的决定簇再刺激产生的。尽管如此，它们的刺激作用被单克隆抗体TU 39抑制，该抗体优先阻断SB相关决定簇而非Dw/DR相关决定簇的刺激。尽管这些克隆具有OKT4 +、OKT -、Leu8 - 表型，并且在与刺激细胞接触后分泌白细胞介素 - 2，但它们在混合淋巴细胞培养中强烈抑制克隆的PLT试剂以及未启动淋巴细胞的增殖反应。因此，它们可能代表一种新型的免疫调节性T细胞，由SB相关抗原刺激产生，尽管具有“辅助/诱导”表型，但能够直接抑制淋巴细胞增殖反应。
