Thesleff I, Ekblom P
J Oral Pathol. 1984 Feb;13(1):85-96. doi: 10.1111/j.1600-0714.1984.tb01404.x.
The nature of the tumor cells in 5 cases of ameloblastomas was studied by immunohistochemistry, and the findings were compared with developing mouse and human teeth as well as with 5 cases of carcinomas in the oral region. The antigens investigated were keratin, an intracellular cytoskeletal protein typical of epithelial cells, and laminin, an extracellular matrix protein found in basement membranes. Our results show that keratin is expressed by all types of epithelial cells in ameloblastomas as well as in the epidermoid carcinomas, and developing teeth. The epithelial, keratin-positive tumor islands in the ameloblastomas were surrounded by a continuous line of laminin, in a pattern similar to that seen in developing tooth. Laminin was seen also around the epidermoid carcinomas but large areas devoid of laminin were constantly seen between the stroma and the neoplastic epithelium. This indicates a lack of proper basement membrane formation by the malignant epidermoid carcinomas. This may be due either to a diminished production or an increased degradation of basement membrane proteins by the carcinoma cells. Our results are in line with suggestions that ameloblastomas are derived from odontogenic epithelial cells. Immunostaining for keratin does not distinguish between carcinomas and the ameloblastomas. However, visualization of basement membrane proteins such as laminin can apparently be used in the differential diagnosis between ameloblastomas and carcinomas.
采用免疫组织化学方法研究了5例成釉细胞瘤中肿瘤细胞的性质，并将结果与发育中的小鼠和人类牙齿以及5例口腔区域的癌进行了比较。所研究的抗原是角蛋白（一种上皮细胞典型的细胞内细胞骨架蛋白）和层粘连蛋白（一种存在于基底膜中的细胞外基质蛋白）。我们的结果表明，角蛋白在成釉细胞瘤、表皮样癌以及发育中的牙齿中的所有类型上皮细胞中均有表达。成釉细胞瘤中上皮性、角蛋白阳性的肿瘤岛被连续的层粘连蛋白线所包围，其模式与发育中的牙齿相似。在表皮样癌周围也可见层粘连蛋白，但在基质和肿瘤上皮之间经常可见大片缺乏层粘连蛋白的区域。这表明恶性表皮样癌缺乏正常的基底膜形成。这可能是由于癌细胞减少了基底膜蛋白的产生或增加了其降解。我们的结果与成釉细胞瘤起源于牙源性上皮细胞的观点一致。角蛋白免疫染色无法区分癌和成釉细胞瘤。然而，诸如层粘连蛋白等基底膜蛋白的可视化显然可用于成釉细胞瘤和癌的鉴别诊断。
