Heikinheimo K, Morgan P R, Happonen R P, Stenman G, Virtanen I
Department of Oral Pathology, University of Turku, Finland.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1991;61(2):101-9. doi: 10.1007/BF02890411.
The distribution of two cellular fibronectins (cFn), tenascin, laminin, as well as type VII collagen was studied in 14 benign odontogenic tumours of epithelial (ameloblastoma) and epithelial-ectomesenchymal (ameloblastic fibroma) origins, as well as in developing human teeth by immunocytochemical means using monoclonal antibodies (Mabs). An extradomain sequence-A-containing form of cFn (EDA-cFn) was seen in the extracellular matrix (ECM) of all tumours studied and in the mesenchyme of the developing tooth germs, indicating that cFn in these tissues are predominantly produced locally. A form of cFn containing an oncofetal domain (Onc-cFn), hitherto found only in carcinomas, was detected focally in the stroma of most ameloblastomas but was absent from ameloblastic fibromas and tooth germs. Tenascin was strongly expressed in the basement membrane (BM) zone of all odontogenic tumours and in that of the early tooth germs. Focal absence of laminin and type VII collagen from the BM of some ameloblastomas and the presence of Onc-cFn in the ECM of most ameloblastomas may correlate with their aggressive behaviour. The results also suggest that EDA-cFn and tenascin are involved in epithelial-mesenchymal interactions during tooth development and in odontogenic tumours.
采用单克隆抗体，通过免疫细胞化学方法，研究了两种细胞纤连蛋白（cFn）、腱生蛋白、层粘连蛋白以及Ⅶ型胶原在14例源于上皮（成釉细胞瘤）和上皮-外胚间充质（成釉细胞纤维瘤）的良性牙源性肿瘤以及发育中的人牙中的分布情况。在所研究的所有肿瘤的细胞外基质（ECM）以及发育中牙胚的间充质中均可见到含额外结构域A的cFn形式（EDA-cFn），这表明这些组织中的cFn主要是在局部产生的。一种含癌胚结构域的cFn形式（Onc-cFn），此前仅在癌组织中发现，在大多数成釉细胞瘤的基质中呈局灶性检测到，但在成釉细胞纤维瘤和牙胚中未发现。腱生蛋白在所有牙源性肿瘤的基底膜（BM）区以及早期牙胚的基底膜区均有强烈表达。一些成釉细胞瘤的BM中局灶性缺乏层粘连蛋白和Ⅶ型胶原，而大多数成釉细胞瘤的ECM中存在Onc-cFn，这可能与其侵袭性行为相关。结果还表明，EDA-cFn和腱生蛋白在牙齿发育过程以及牙源性肿瘤中参与上皮-间充质相互作用。
