Tokita H, Tanaka N, Ueno T, Okamoto K
Gan No Rinsho. 1983 Dec;29(15):1755-60.
To evaluate the time response of anticancer drugs, we observed the morphological changes of solid tumor cells using a new simple method of cytologic examination. Mitomycin C and Bleomycin were administered to nude mice transplanted with human uterine cervical carcinoma of the Yumoto strain. The cancer cells were classified as follows: A, uniform, pyknotic nuclei with indistinct nucleoli; B, pyknotic nucleus with distinct nucleoli; C, pale-stained nuclei and cytoplasm; D, condensation of nuclei and cytoplasm. Three hours after MMC administration the destruction of the nuclei of A type cancer cells and shrinkage of B type cells was observed. Pyknosis of B type cells and destruction of C type cells was seen 7 hours after BLM administration. These characteristic changes which occurred at a very early period appear to be available as a marker for the evaluation of the oncostatic effect and for the anticancer drug selection in patients with malignancy.
为评估抗癌药物的时间反应，我们使用一种新的简单细胞学检查方法观察实体瘤细胞的形态变化。将丝裂霉素C和博来霉素给予移植有尤本托株人子宫颈癌的裸鼠。癌细胞分类如下：A，核均匀、固缩，核仁不明显；B，核固缩，核仁明显；C，核和细胞质淡染；D，核和细胞质凝聚。给予丝裂霉素C三小时后，观察到A型癌细胞核破坏和B型细胞收缩。给予博来霉素7小时后，可见B型细胞核固缩和C型细胞破坏。这些在很早时期出现的特征性变化似乎可用作评估抑癌效果和为恶性肿瘤患者选择抗癌药物的标志物。
