Thomander L, Aldskogius H, Grant G
ORL J Otorhinolaryngol Relat Spec. 1984;46(2):111-6. doi: 10.1159/000275695.
The intraaxonal transport of horseradish peroxidase, applied to the proximal stump of different transected hypoglossal and facial nerve branches, was used to label fibers in the hypoglossal main trunk and in the intratemporal portion of the facial nerve in the rat. By using tetramethylbenzidine as a substrate, numerous heavily labeled fibers are obtained. In longitudinal sections the arrangement of fibers can be studied for long distances. From these sections, sites of specific interest can be further examined in transverse-like reconstructions of the nerve. Thus, the procedure described offers a convenient way of analyzing the intrinsic organization of peripheral nerves.
将辣根过氧化物酶应用于不同横断的舌下神经和面神经分支的近端残端，利用其轴突内运输来标记大鼠舌下神经主干和面神经颞内部分的纤维。通过使用四甲基联苯胺作为底物，可获得大量标记强烈的纤维。在纵切面上，可以对纤维的排列进行长距离研究。从这些切片中，可以在神经的类似横切的重建中进一步检查特定感兴趣的部位。因此，所描述的方法为分析周围神经的内在组织提供了一种便捷的方式。
